FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492836
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC81860.3283810086182966No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT54870.2201107493633757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50570.20286131939686367No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC39880.15997843420104652No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT30340.1217087686474361No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT29600.1187402620950596No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT29580.1186600321882386No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA26200.10510117793549195No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA25350.10169140689560002No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG24940.10004669380576982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT14550.040.3145561
ACTCTAG17900.034.5058985
GACTCTA17750.033.4333464
CTGACTC18900.031.7628752
CTAGCAG20050.029.0830238
TGACTCT24150.024.8588983
CTCTAGC24300.024.8504246
TAGCAGA24050.024.3893959
CTATCGA26300.021.7750217
TCTATCG27000.021.72157916
GATCTAT28400.020.65038314
TATCGAT28650.019.98893518
ATCTATC29400.019.71330515
GCAGATC31550.019.3563611
AGATCTA31000.018.69584713
TCTAGCA49400.018.0202167
TAGCATG32000.018.00379227
ATTCTAG32400.017.78152323
AGCAGAT34250.017.62898310
CTAGCAT33350.017.27500326
Produced by FastQC (version 0.11.2)