FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615031
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT34680.21473272030072488No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28290.17516691630067782No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA19050.11795439220671307No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG17760.10996692942736085No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC17390.10767595173095748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG8200.017.6731431
CGTTCAC6850.017.12430466
GGCCGTT6550.016.85465263
CGTGGCC6850.016.61961760
GCCGTTC6950.016.38099364
GGATAAC17950.015.7625951
CCGTTCA7550.015.53661965
CAAGGGC8600.015.2443762
GCGGGAT2554.5877096E-714.8843821
CGAACGC4950.014.63564160
GTTCACG8050.014.5716167
TCGTGGC7900.014.41068259
CGCGCCG5050.014.34627164
AACGCGC5200.013.93200562
GAACGCG5450.013.92591961
GATAACA22450.013.3698792
TCACGGA8550.013.31636969
TGGCCGT8350.013.22090562
CGTAGGG9150.013.19607121
GGGGCAT1850.00172414313.0558351
Produced by FastQC (version 0.11.2)