FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_jes_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_jes_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10056185
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG702500.6985750560475966TruSeq Adapter, Index 5 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281280.2797084580285665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC87650.059.67133744
TATGCCG87800.059.6069146
TCGTATG92150.056.68391443
ATGCCGT92250.056.58225647
GTATGCC92950.056.48989545
GCACACG109450.055.73003411
ACGTCTG110150.055.342915
CACACGT111050.054.8946512
CTCGTAT97650.052.85531242
ACACGTC116150.052.48377213
CACGTCT118400.051.515814
CACAGTG103350.050.97456733
GCCGTCT103750.050.31023849
TGCCGTC105150.049.509448
AGCACAC124800.049.18024410
CACACAG109200.048.02286531
GTCACAC110150.047.79614329
TCTCGTA108200.047.5101241
ACACAGT110350.047.49090232
CGTCTGA131550.046.5244516
Produced by FastQC (version 0.11.2)