FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_jes_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_jes_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10187327
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG776080.7618092557547235TruSeq Adapter, Index 4 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288610.28330297044553493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG91300.059.5914546
CGTATGC91600.059.54810744
GCACACG115050.056.65005511
GTATGCC96500.056.4163345
ACGTCTG115950.056.11473515
ACTGACC98000.055.76294732
CACACGT117100.055.71177712
ATGCCGT98200.055.1921247
TCGTATG98450.055.05466543
ACACGTC120800.053.94746413
CTCGTAT100400.053.47044842
CACGTCT125300.051.9822114
CACTGAC107100.051.2501631
TGCCGTC107950.050.33353848
GCCGTCT109100.049.9927149
AGCACAC130700.049.94622410
TCTCGTA108900.049.29690641
CGTCTGA132950.049.0435116
TCCAGTC138350.046.77902225
ACTCCAG139750.046.63222523
Produced by FastQC (version 0.11.2)