FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_jes_123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_jes_123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24815065
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1229520.49547321354991414TruSeq Adapter, Index 8 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG596550.24039832255124052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG161550.056.50673346
CGTATGC163450.056.084244
GTATGCC171400.053.3003145
ATGCCGT175450.052.1665947
ACGTCTG208950.051.56268715
GCACACG209000.051.45483811
TCGTATG177950.051.4376443
CACACGT216300.049.74725712
CTCGTAT185750.048.7763742
CACGTCT227950.047.24981314
ACACGTC229900.046.8187513
GCCGTCT200500.045.69971549
AGCACAC243650.044.33646810
TGCCGTC210200.043.5418648
TCTCGTA210650.043.10891741
GTCACAC216150.042.775529
TCCAGTC255400.041.8602925
CTGAACT260750.041.3330919
CAGTCAC229050.041.25398627
ACTCCAG263700.040.673823
Produced by FastQC (version 0.11.2)