FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_jes_122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_jes_122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10176481
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG537850.5285225806445274TruSeq Adapter, Index 7 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238750.2346095865555097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC72000.057.7431644
TATGCCG72500.057.43746
GTATGCC76550.054.44568645
ATGCCGT78250.053.34787847
TCGTATG78800.053.11156543
ACGTCTG90700.050.778715
GCACACG93450.049.80540511
CTCGTAT84550.049.1323442
CACACGT95450.048.43292612
ACACGTC96300.047.96936813
TGCCGTC86850.047.94424448
GCCGTCT88450.047.03796849
CACGTCT101000.045.70252214
CATCTCG93050.044.75451739
TCTCGTA93900.044.3870141
AGCACAC105950.044.22287410
CGTCTGA105850.043.7390416
AGTCACC100100.041.97790528
ACTCCAG110450.041.4801123
CAGTCAC102250.041.39829327
Produced by FastQC (version 0.11.2)