FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_jes_121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_jes_121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11013976
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG824630.748712363273717TruSeq Adapter, Index 6 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG283030.2569735034832108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC101850.060.7360944
TATGCCG102250.060.39644246
GTCACGC106150.058.4366729
GTATGCC107750.057.34584445
ATGCCGT109700.056.38784447
TCGTATG109950.056.29458243
CACGCCA111500.055.8493531
AGTCACG113400.054.7911628
TCACGCC114000.054.5035330
ACGTCTG127200.054.27149615
CTCGTAT113850.054.2452142
GCACACG128200.053.85231411
TATCTCG115150.053.5723939
CACACGT129400.053.37618312
CGCCAAT117000.052.92907733
GCCGTCT117200.052.8370849
GCCAATA117400.052.6314434
ACACGTC132350.052.18551313
ACGCCAA121250.051.07383332
CACGTCT135550.051.0301314
Produced by FastQC (version 0.11.2)