FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_jes_111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_jes_111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11130113
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG966200.868095409273922TruSeq Adapter, Index 3 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG255970.22997969562393483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG119350.060.84818346
CGTATGC120650.060.223344
ATGCCGT124700.058.1809747
GTATGCC124900.058.03491245
TCGTATG127900.056.8110843
TAGGCAT128600.056.36238535
GCACACG156850.056.2664511
CACTTAG132500.055.6935631
ACGTCTG158000.055.63530715
CACACGT158900.055.47316712
TTAGGCA132450.054.82874334
CTCGTAT130700.054.82871242
ACTTAGG134100.054.66864432
ACACGTC161850.054.46133413
TGCCGTC137150.053.02456748
CACGTCT167350.052.50676714
CATCTCG138100.052.09062239
GCCGTCT139850.052.0746249
TCACTTA142650.051.9968230
AGCACAC170700.051.86428510
Produced by FastQC (version 0.11.2)