FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_jes_110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_jes_110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11226659
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG917590.8173313182488219TruSeq Adapter, Index 2 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323100.28779710865004454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC105350.059.14507744
TATGCCG106750.058.3988646
GTATGCC110700.056.37890645
ATGCCGT112750.055.1680347
TCGTATG113700.054.9231543
ACGTCTG135200.054.86180515
GCACACG136600.054.4539711
CACACGT138100.053.8344112
ACACGTC141400.052.5050613
TATCTCG118650.052.3989339
CTCGTAT119550.051.91811442
CGATGTA119650.051.84242234
CACGTCT143250.051.8026714
CCGATGT119850.051.69833833
TGCCGTC121950.051.11881648
GCCGTCT123550.050.4286749
AGCACAC151550.049.26479710
CGTCTGA154200.048.23701516
TCTCGTA129250.048.12851741
ACCGATG133750.046.7382832
Produced by FastQC (version 0.11.2)