FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_jes_109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_jes_109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9346671
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG390440.41773161802742387TruSeq Adapter, Index 1 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243890.26093782481484584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC52300.055.8729544
TATGCCG52550.055.34424246
GTATGCC57800.050.49690645
ATGCCGT59200.049.3599447
GCACACG69700.048.8565611
TCGTATG60900.048.3230143
ACGTCTG70600.048.0845615
CACACGT71850.047.43982712
CTCGTAT64650.044.55972342
ACACGTC76450.044.5400113
TGCCGTC66500.043.7856148
CACGTCT77950.043.5946314
GCCGTCT68250.042.35915849
AGCACAC82800.041.12771610
TCTCGTA72150.040.11900741
GTCACAT74400.039.41518429
TCCAGTC86000.039.15209625
CGTCTGA87050.039.03756316
CATCACG78400.038.06427833
TCACGAT79250.037.39422235
Produced by FastQC (version 0.11.2)