FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8574499
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7827239.12849835308162No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1443571.683561920060869Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122090.1423873278193863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGA276050.064.8839652
GCACACG145500.062.3960118
ATAACTG289250.062.0141141
ACTGAAG285650.061.3912284
CACACGT103950.061.11260619
ATCGTCC42950.060.4444811
AGAGCAC197850.060.38761515
AGCACAC153000.059.31344217
ACACGTC65550.059.1207520
GATCATC79600.057.56899310
AACTGAA319050.057.2633323
GAGCACA195700.056.66501216
GTGACTG87050.056.3925861
TGACTGG87850.056.011242
GATCCTC37950.055.39026310
CTGAAGA345100.054.961595
CACGTCT38300.054.8009521
CACACGA42950.053.92570519
ATCATCC63850.053.66356711
GCACGCG20900.053.4806718
Produced by FastQC (version 0.11.5)