FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131792
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37200.17450107702815285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC16250.038.5972335
TCGCGAG1550.031.6783052
AAAAGGG64700.029.68836270
CAAGATC21950.029.5320595
CGCGAGA2850.029.5207523
CTAGATC13200.028.668595
CGCGCAG1253.4688128E-928.0711541
CATTAAA160200.028.0353761
CCGTATC162550.027.99859455
CGCGGAC1750.027.95453349
CGTATCA164850.027.71389456
TCATTAA158800.027.6007660
GCGAGAT7200.027.2573474
GTATCAT159450.027.09589857
GCCGTAT174500.026.98257454
CGCCGTA172050.026.9423753
TCGCCGT171950.026.83677752
CCGAGAT6050.026.6459084
ATCATTA168200.026.03750259
TATCATT169200.026.02871558
Produced by FastQC (version 0.11.5)