FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5839594
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99650.17064542500728647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC25950.025.5235
TGCGGTG11050.025.0847511
CGCGAAG8500.023.1160761
CGCGGAC6100.022.33939249
AAAAGGG130900.022.19942370
CATTAAA283200.020.53355461
TCATTAA282150.019.92932960
TGCGTCG10100.019.4442542
GTATCAT281800.019.40991857
CGTATCA298150.019.38813656
CCGTATC296350.019.34188855
CTAGATC27600.018.9184025
ATCATTA303050.018.47453559
TGTCGTG16350.018.4554961
TATCATT307250.018.26762258
GCGAGAT13450.018.2405954
CGCCGTA321900.018.18926653
GCCGTAT327400.018.10592354
TCGCCGT328400.017.85067752
CGGCAAG18150.017.7850951
Produced by FastQC (version 0.11.5)