FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12805985
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206540.16128396214738655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG34700.021.9417131
CCGTATC289550.020.49518655
CGTATCA297900.019.9317456
CGCCGTA310850.019.5877553
CGAGATC34900.019.578645
TCGCCGT316650.019.01965352
GCCGTAT324750.018.99505454
CGCGAAG17100.018.4665891
GTATCAT312800.018.23389457
GTCGCCG341900.018.2084151
TGTCGGC26600.018.05380830
GGTCGCC347800.018.04078950
CGGGCAG12350.017.8983861
CGGCCAG10000.017.8941251
CATTAAA341600.017.8206161
TGGTCGC350550.017.772249
CGAGCAG32900.017.1699981
TCATTAA341600.017.14581960
TGCGGTG23600.016.9485721
AGGGGTG45250.016.903561
Produced by FastQC (version 0.11.5)