FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8943838
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG194320.21726690487909103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACC28300.060.568473
TCGTGAC30800.056.5785332
GTGACCT32300.053.7077184
TTCGTGA41350.045.898081
TCGCGAG11300.042.8200042
CGCGAGA20450.038.566783
CGAGATC140050.037.5055
TCGCGTA8150.036.157171
GCGAGAT51450.035.1478164
AAAAGGG619550.035.09091670
CTAGATC114900.034.583085
GCTAGAT69250.033.7045484
CAAGATC170500.033.5813675
AAAGGGG275050.033.3618370
TTCGCGA9050.033.3367041
CGCTAGA27850.033.22793
CGTAGAT55900.032.1617434
CGCGTAG10050.031.7483652
CCGAGAT31100.031.2142874
CATTAAA1321800.030.76229361
Produced by FastQC (version 0.11.5)