FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4171657
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76560.18352419674004838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC24450.029.092115
AAAAGGG104300.026.5580770
CGCGAAG7200.026.3046251
CGGCTAG3000.025.7200761
CATTAAA223150.025.02930361
CGTATCA224650.024.91151656
CCGTATC224400.024.86172955
TCATTAA218600.024.75132660
GTATCAT213350.024.72424957
CTAGATC23800.024.1448755
CGCCGTA243000.023.85262753
TCGCCGT245400.023.52110152
GCCGTAT248600.023.4538954
ATCATTA232900.023.15688959
TATCATT236500.022.96741158
CTCCGTG6900.022.81810844
TCGCGAG2800.022.5354272
GTCGCCG264050.022.31022651
GGTCGCC269200.022.11704450
TGGTCGC269100.021.88092249
Produced by FastQC (version 0.11.5)