FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15480492
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2162241.3967514727568089Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1769821.1432582375288847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG76450.050.27431518
AGAGCAC131250.047.00598515
GAGCACA128050.046.26623516
AGCACAC99150.042.82680517
CACACGT59300.040.35432419
AAGGGGG365750.036.01735367
AAAGGGG383000.034.44987566
AGGGGGG418500.033.68062668
ACACTCT71750.031.5449281
AAGAGCA218150.030.816514
ACACGAG38300.029.54817620
CACACGA41700.029.32334119
CCGTATC749100.029.29963549
CGTATCA764000.028.83069650
CGCCGTA766300.028.81264547
CAGATCG269350.028.7048956
GCCGTAT782450.028.54885548
TAGATCG272000.028.2191586
TCGCCGT771600.028.06464446
GTATCAT762300.028.01372151
Produced by FastQC (version 0.11.5)