FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10555611
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1144711.084456409013178No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC721020.6830679910428681Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG42100.048.18662618
GAGCACA71350.042.5233116
AGAGCAC73250.042.1847915
AGCACAC56500.041.54701617
CACACGT34750.038.1131919
AAGGGGG145450.030.42096167
TAGATCG122850.029.2286366
AAAGGGG152650.029.12323266
CAGATCG133800.027.883896
AGGGGGG173100.027.7002468
GATCGGA497300.024.9785798
AAGAGCA132300.024.91652914
CCGTATC306750.024.75148849
AGATCGG502400.024.7258387
CGCCGTA311050.024.66427847
CGTATCA313950.024.33688750
GCCGTAT320050.024.27141648
TCGGAAG510200.024.1745610
TCGCCGT311900.024.04189346
AAGATCG129650.024.020896
Produced by FastQC (version 0.11.5)