FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13322706
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1406951.0560542280224452No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC509920.38274506695561694Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG56200.049.2468618
AGAGCAC96350.041.12075415
GAGCACA93900.040.77693616
CACACGT53750.040.2163519
AGCACAC79150.040.14496617
ACACGTC34950.030.87470420
TAGATCG121200.030.0857416
GATCGGA441350.029.136028
CAGATCG123000.028.9619946
AGATCGG444450.028.9564427
CGCCGTA251600.028.1473453
TCGGAAG455450.028.06385810
GGTCGCC261750.027.91241650
TCGCCGT254950.027.5314452
ATCGGAA466850.027.4317079
GTCGCCG264550.027.40505651
GCCGTAT263200.027.34218654
CCGTATC256450.027.2160555
GTGGTCG271400.026.74443448
GAGATCG94050.026.7410376
Produced by FastQC (version 0.11.5)