FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19837342
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2198751.108389420316492No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC268260.13522981052602712Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG84400.051.05914318
CACACGT83850.043.3717919
AGAGCAC133900.041.5744715
AGCACAC117950.041.13912217
GAGCACA135500.039.24914616
ACACGTC58950.031.14324420
GATCGGA327900.029.1657288
AGATCGG331100.028.7573937
TCGGAAG349050.027.44811210
ATCGGAA363950.026.1994049
TAGATCG103900.025.657556
CAGATCG117200.025.2865056
AAGAGCA238600.024.84208714
CGGAAGA381850.024.74184211
GAAGAGC404750.023.28205913
GGTCGCC140550.020.92883950
CGCCGTA137200.020.63440547
CACGTCT48900.020.56313921
GGAAGAG471350.020.4899812
CGGGGAC16200.020.3580381
Produced by FastQC (version 0.11.5)