FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6439554
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC122878019.08175628312147Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG925651.4374442702087753No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC316640.49171107191585006Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC221640.3441853271204807Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT213730.3319018677380452Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG172530.2679222815741587Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC168800.26212995496271946Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC162850.25289018463079893Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCC140150.21763929613758964Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT123180.19128653940940632Illumina Single End PCR Primer 1 (100% over 43bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC120280.18678312193670554Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC119060.18488858079301765Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTAGCC112820.17519846871382708Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC103210.16027507495084287Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC101650.15785254693104522Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC90660.1407861476120862Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC80720.12535029599876016Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTTGTCGCC79340.12320729044278532Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG79190.1229743550562663Illumina Single End PCR Primer 1 (100% over 42bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCTCGGTGGTCGCC73040.11342400420898714Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG72990.11334635908014747Illumina Single End PCR Primer 1 (100% over 41bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC72260.11221274019908832Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC70040.10876529647860707Illumina Single End PCR Primer 1 (100% over 38bp)
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC67850.1053644398354296Illumina Single End PCR Primer 1 (100% over 38bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC64980.10090760944003266Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAATAGTGTAGATCTCGGTGGTCGCC64430.1000535130227963Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA2050.070.155181
CCTGTGA700.070.155181
CTGCCTA1050.070.155181
ATGCGCA1000.070.155181
TTGCCTA850.070.155181
CCTGCCA1050.070.155181
CTGGCCA1600.070.155181
GCACTGA700.070.155181
CACCCCA800.070.155181
CGGACCA2350.070.155181
TCCCTGA150.002205912770.1551741
GCCCAGC150.002211922570.107073
CGCAGAC150.002213905870.0912254
GTCAGGT301.01737896E-770.07593524
ACACTCT140650.069.332181
CCGGGCA5300.068.83151
CCCGCCA2400.068.693611
CACTCTT133250.068.647812
CCGCCCA2250.068.5961761
CGCTGCA2000.068.4013061
Produced by FastQC (version 0.11.5)