HMWVKAFXY_n02_ncm3-14.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HMWVKAFXY_n02_ncm3-14.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7773395
Sequences flagged as poor quality	0
Sequence length	76
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	1269853	16.33588670072729	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	113377	1.4585261652083805	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	26023	0.3347700715067226	Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	23581	0.3033552263843533	Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	22757	0.29275496742414353	Illumina Single End PCR Primer 1 (100% over 39bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	20161	0.25935900594270583	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC	18386	0.23652471024565197	Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	17910	0.23040125968125896	Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	17884	0.23006678549076692	Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC	15269	0.1964264005624312	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC	14229	0.18304743294274894	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCC	13873	0.17846770941139617	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCC	11740	0.15102796139910554	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCTCGGTGGTCGCC	11085	0.1426017846770941	Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	10898	0.1401961433839397	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	10390	0.1336610322774026	Illumina Single End PCR Primer 1 (100% over 43bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC	10292	0.1324003218670864	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC	10272	0.13214303402824634	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTAGCC	9599	0.12348529825127888	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATGTCGGTGGTCGCC	9484	0.12200589317794862	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC	9081	0.11682154322532176	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGCGGTGGTCGCC	8920	0.11475037612265941	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTTGGGAAAGAGTGTAGATCTCGGTGGTCGCC	8718	0.11215176895037497	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	8226	0.1058224881149099	Illumina Single End PCR Primer 1 (100% over 41bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	8033	0.10333966047010347	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCC	7914	0.10180879782900523	Illumina Single End PCR Primer 1 (98% over 50bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCGA	40	1.3278623E-10	70.14744	1
GCCCGCA	440	0.0	69.3503	1
ACACTCT	16450	0.0	69.18797	1
GGCACCA	320	0.0	69.051384	1
CCGGCCA	510	0.0	68.77199	1
CCCGCCA	230	0.0	68.62249	1
CACTCTT	15750	0.0	68.40621	2
CGCCTCA	190	0.0	68.30145	1
GGCCGCA	510	0.0	68.084274	1
GTGACTG	10655	0.0	68.00779	1
CAGGCCA	320	0.0	67.95533	1
CCTGCAA	145	0.0	67.72856	1
CCGCGCA	570	0.0	67.68612	1
CGGCCCA	545	0.0	67.57322	1
CGCGCCA	540	0.0	67.54938	1
CCGGGCA	745	0.0	67.32271	1
GCTACAG	345	0.0	67.07155	2
ACGGGCA	430	0.0	66.884766	1
CAGGCTA	215	0.0	66.884766	1
CCCCTCA	105	0.0	66.807076	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content