FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7648852
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC116930015.287261408640147Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG969301.2672489937051992No Hit
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC378480.4948193532833424Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT365920.47839858844176875Illumina Single End PCR Primer 1 (100% over 39bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC317810.41550026069271573Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG238310.3115630946970866Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC210980.27583224253783445Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC165070.21581016340752834Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCC144830.18934867611505624Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC123220.1610960703645462Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC119210.15585345356401195Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTAGCC112210.14670175341345343Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG112190.14667560569873753Illumina Single End PCR Primer 1 (100% over 41bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC109950.1437470616505588Illumina Single End PCR Primer 1 (100% over 49bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC107660.14075314831559035Illumina Single End PCR Primer 1 (100% over 38bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC101970.13331412347892208Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC92410.1208155158447307Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT89710.11728557435808667Illumina Single End PCR Primer 1 (100% over 43bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC83440.10908826579465782Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC82700.10812080035017019Illumina Single End PCR Primer 1 (100% over 49bp)
ACACTCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG82500.10785932320301138Illumina Single End PCR Primer 1 (100% over 42bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC80840.1056890628815932Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTTGTCGCC79700.10419864314278797Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCG150.00221457470.0860226
ACACTCT221800.069.072831
TAACTGA105450.069.058392
CGGCCCA6250.069.025771
CGCGCCA5300.068.824591
GCGTGCA2200.068.553861
ATAACTG106750.068.37391
CACTCTT215550.068.365652
GTGACTG123650.067.963991
GCCATAG5550.067.595432
CCCGCCA2500.067.342221
CGGGCCA6100.067.273221
GCCGCCA4650.067.131011
CAGCGCA3200.066.859941
CGGACCA3100.066.7538761
GCCCGCA4100.066.726281
CGTAGCG4050.066.628924
CGCATAG3950.066.571862
ATCATCC26750.066.39184611
GCGCTAG10000.066.2656252
Produced by FastQC (version 0.11.5)