FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4629459
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90200.1948391809928547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC36100.041.548945
GCGAGAT16300.036.126434
AAAAGGG141700.031.35130770
CTAGATC30850.030.6712845
TCGCGAG4350.030.6360822
CAAGATC49150.029.3762975
CGCGAGA7250.029.0164153
CATTAAA338450.029.01619161
TCATTAA339400.028.26613660
CCGTATC348050.028.25965955
CGTATCA352600.028.09189856
GTATCAT337200.027.82118257
CTCGCGA2400.027.7750951
CGCCGTA376000.027.1765153
GCCGTAT381500.027.0098554
CGCGCAG2600.026.9879461
TCGCCGT378150.026.94954552
ATCATTA356900.026.78345959
TATCATT365400.026.20848858
GTCGCCG404800.025.94568451
Produced by FastQC (version 0.11.5)