FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218954
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28200.23134589164152214No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCC450.00434462431.05816351
CGCGAAG2100.030.0698781
CGAGATC7350.028.1305395
CGGGCAT1154.7955837E-827.4551071
CAAGATC10050.025.1061345
CGAGCAG2800.025.0582331
CGCGGAG1001.37385905E-524.5570661
CCGTATC55900.023.74614755
TTCGGCT750.00181844123.3655430
GTATCAT55600.023.30787357
CGTATCA58450.023.24709156
GCGAGAT3800.023.0580984
CATTAAA59500.022.9533261
TTCAGCG3250.022.668061
CGCCGTA61600.022.57422453
TCATTAA58900.022.4162160
CGGCCAA1102.871577E-522.3246061
CTAGATC6750.022.3243145
CGGCAAG3150.022.2739831
CGCGCTG952.738102E-422.1567521
Produced by FastQC (version 0.11.5)