FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n02_ncm3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n02_ncm3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243878
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42110.1876661743642034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC14550.034.926325
CGCGAGA3850.028.2264083
AAAAGGG62950.027.34754270
TGCGTCG5250.026.0650481
CATTAAA148150.026.02163361
GCGAGAT6500.025.8824144
TCGCGTC959.222465E-625.8539961
TCGCGAG2050.025.6594642
CAAGATC22200.025.416795
CGAGCAG4900.025.0625461
TCATTAA149800.025.0123860
CGGGCAG1902.1827873E-1124.0072821
CGTATCA162650.023.89613256
CCGTATC161650.023.72097455
GTATCAT154400.023.6118557
CTAGATC14150.023.5296215
ATCATTA159200.023.31617559
CCGAGAT4400.023.1005464
TATCATT161650.022.89798758
CGCGCAG1553.4207915E-822.637141
Produced by FastQC (version 0.11.5)