FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8574499
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1980872.3101874523514434TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG475000.5539682260153043No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG165610.1931424798113569TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG162440.1894454708082653TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCA98670.1150737786545896TruSeq Adapter, Index 5 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG106050.042.5529670
GTGACTG54300.040.4564441
TGACTGG56100.039.6502722
AAAAAGG266850.034.89727870
ACTGGAG62700.034.6398474
CCCGATC23950.032.7731737
GACTGGA67300.032.5843
GGATCTC24500.032.16486739
TGATCGG33000.031.0585781
GAGTTGG35550.030.9828628
GCCCGAT9750.030.54852736
CTTGAAA355300.030.49477661
GCTTGAA339350.030.4532560
CTGCTTG329650.029.5773158
TGCTTGA331550.029.05939159
AAAGGGG29500.028.69750270
TTGAAAA394100.028.66440662
CACTTAC33550.028.40726932
GTCACAG655850.028.40690830
ACGTGGG34950.028.2132137
Produced by FastQC (version 0.11.5)