FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131792
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT22300.049.931411
GCTTGAA134250.034.80366566
TGCTTGA132200.033.99316465
GAAAAAA157450.033.78772470
CTTGAAA139700.033.74653667
CTGCTTG135950.033.0812564
TGAAAAA158950.032.76426369
GCGTGAA12100.031.81744466
TTGAAAA152300.031.23040268
TCTGCTT147900.030.45568763
CACTCTT53050.028.9754851
TTCTGCT154000.028.95387862
CGTGAGA4000.028.8811073
CTTCTGC164550.028.8416361
GCGAGAT8800.028.642424
CGTGAAA13700.028.1015467
GAGATCG74450.027.9306876
GTCTTCT170350.027.71582259
CTGCGTG10150.026.55111164
TGCGTGA10600.026.41448465
Produced by FastQC (version 0.11.5)