FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5839594
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT62700.046.851831
GCCGGCT12750.031.29334856
CGGCTTG21750.029.930358
TGCCGGC25050.029.34059755
CACTCTT148350.028.7233561
GCGGCTT18950.027.70380257
CCGGCTT21500.027.67373857
GCTTGGG12800.027.61650760
GCGTGAA18800.027.36634866
CCGGCGT20350.026.6578257
CGGCGTC15100.026.1914158
GCGGCGT21400.025.34984457
GCGTGGG22300.025.26843660
CGGCGTG27950.025.04410758
CGTGAAA24650.024.56329267
TGGCGGC26150.023.95734655
GCGTCGG10000.023.79941660
GCCCACA17800.023.2110181
GGGTGGA22750.022.92251466
GGCGTGA11050.022.8048765
Produced by FastQC (version 0.11.5)