FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12805985
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT76150.034.2559551
GCGTGAA38100.026.73156466
GCCGGCT26800.025.59632756
TGCCGGC43200.025.0340555
GCCCACA31100.023.9810921
CCGGCTT42050.021.30739457
CGTGAAA53800.021.01244567
TGCGTGA29350.020.86826165
CCGGCGT27900.020.82381457
GCGTGGG22900.020.78546760
CACTCTT172750.019.8230531
CGGCTTG32100.019.62563958
ATGCCGG38450.019.11522154
GGCGTGA20000.018.89949265
ATCGCGT69700.018.72976947
CATCGCG125700.017.87541646
GCGGCGT24700.017.71207457
CGGCGTG36200.017.5962158
GGGGGGA25900.017.56709566
GAGATCG135950.017.4852056
Produced by FastQC (version 0.11.5)