FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8943838
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT114950.12852424205357924TruSeq Adapter, Index 6 (100% over 39bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA103250.11544260976104441TruSeq Adapter, Index 6 (100% over 38bp)
CTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA100990.11291573036094796TruSeq Adapter, Index 6 (100% over 40bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA96790.10821975979439699TruSeq Adapter, Index 6 (100% over 37bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT91510.10231625393930437TruSeq Adapter, Index 6 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACG31950.057.7298612
ACACTCT162850.057.6821941
AGGTCAC35350.052.6723811
TCACGAA43500.042.16020614
GGCGTGA99000.039.1710665
GAAAAAA1204250.038.94474470
CGGCTTC91300.038.7945358
GCGTGAA264700.038.78098766
CGTGAAA273250.038.66906467
GCCGGCT94700.038.62131556
CCGGCTT102850.038.52147357
CACGAAA49350.037.87170415
ACGAAAG49900.037.52441816
TGAAAAA1176400.037.29918369
GTGAAAA317750.037.009668
GCTTGAA807300.035.8231366
CTTGAAA842200.035.3983667
TGCGTGA174350.035.23026365
CACTCTT402550.034.9461171
GCGTCGG32150.034.83611760
Produced by FastQC (version 0.11.5)