FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4171657
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT37250.045.8659481
GCCGGCT23000.041.0863356
GCGTGAA36050.040.19356566
CCGGCTT32500.037.90711257
CGGCTTC27700.037.273858
CGTGAAA42400.036.7329367
TGCGTGA23500.036.33987465
CTGCGTG23900.034.1208264
GGCGTGA15300.034.08444665
TGCCGGC42650.033.9736955
GCGTGGG14250.031.19250760
GTGAAAA69150.030.8744468
GAAAAAA174500.029.18293670
CCGGCGT23750.029.17850357
GGCTTCG10600.029.0561659
GCCGTCT36600.028.49683456
CACTCTT92200.027.9475441
TTCTGCG24450.027.91368762
ATGCCGG45300.027.73688554
TCGGCGT17150.027.55060263
Produced by FastQC (version 0.11.5)