FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15480492
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG975330.6300381150676606TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGTATG190230.12288369129353253TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATT183380.1184587673311675TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT68000.032.11699356
CCGGCTT89200.031.11483857
CTGCTTG227300.031.08823858
TGCCGGC110350.030.22593955
GCGTGAA134350.028.81221866
ATGCCGT290850.028.69977648
GGCGTGA54050.028.16779165
TGCCGTC281900.027.798349
CTCGTAT378650.027.39677643
CATCTCG503100.027.3191540
TCTCGTA392300.027.1750842
AAAAGGG193650.026.80296970
GCCGTCT215900.026.79658550
TGCTTGA248650.026.7720359
AAAAAGG464900.026.67293770
CGTCTGC100300.026.55479455
TATGCCG336800.026.48847847
GCTTGAA287100.026.45365760
GTATGCC370600.026.4241846
TCTGCTT304800.026.226557
Produced by FastQC (version 0.11.5)