FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10555611
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT37950.029.32730156
TGCCGGC61250.028.85639455
CCGGCTT52400.028.78742257
GCGTGAA63350.026.96062766
GGATCGC118400.026.5153645
ATCGCGT77600.026.15911547
GATCGCG111600.026.09251446
GGCGTGA26050.025.52716865
TCGCGTA60450.025.12755248
ATGCCGG59150.024.55557654
GAGATCG93500.024.3373766
CGGCTTC52600.024.21990658
ATGCCGT95100.024.10557748
CCGGCGT36050.023.9799657
CGCGTAT67250.022.95107849
CTGCTTG92000.022.86353758
TGCCGTC91550.022.8612349
GATCTCG136550.022.63214940
GCGTGGG25600.022.42129560
GGATCTC143550.022.35748339
Produced by FastQC (version 0.11.5)