FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13322706
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG539160.40469256020511146TruSeq Adapter, Index 22 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT281700.024.8113956
TATGCCG317250.024.24855853
TGCCGTC311050.023.8767455
GAGATCG95150.023.729796
ATGCCGT316600.023.49134354
GTATGCC329950.023.45311252
CCGTCTT305200.023.27937757
CCGTACG342200.023.05333540
CGTACGA330250.022.98669641
CGTATGC333150.022.94418751
GATCTCG274900.022.80240646
CGATCTC277200.022.7773545
CTCGTAT307500.022.44510549
TACGATC297450.022.1797843
ACCGTAC356900.022.01555639
TCGTATG335600.021.94237550
ATCTCGT287150.021.9149647
TCTCGTA297350.021.83412648
GTACGAT326350.021.81359142
AGATCGG421950.021.7984627
Produced by FastQC (version 0.11.5)