FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19837342
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGC34950.016.12270755
CACGTGG199050.015.47318738
AGTCACG210600.015.40567235
CGTGGCC200300.015.28925740
ACGTGGC202550.015.24037539
TCACGTG210550.014.89402337
GTCACGT217600.014.73315836
GCACACG256650.014.53701518
ACACGTC263750.014.21203620
GATCGGA263500.014.1591078
AGATCGG265600.014.15257557
CACACGT264300.014.12949219
ACGTCTG267400.013.9264222
GCCGGCT24750.013.85830656
GTGGCCA215700.013.84070341
CGTCTGA268050.013.80125123
CACGTCT273000.013.71767221
AGCACAC274700.013.58181617
CAGTCAC241900.013.5714534
GAACTCC262300.013.54341428
Produced by FastQC (version 0.11.5)