FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6439554
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG122791119.068261559729134TruSeq Adapter, Index 4 (100% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC310270.4818190825016763TruSeq Adapter, Index 4 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA307110.47691191035900937TruSeq Adapter, Index 4 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG306740.4763373364055958TruSeq Adapter, Index 4 (97% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG273630.4249207320879676TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATG267970.4161313035033172TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCACTCTCGTATG264830.411255189412186TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATT251550.3906326431923702TruSeq Adapter, Index 4 (97% over 48bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA223050.34637491975375934TruSeq Adapter, Index 4 (100% over 39bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA218470.33926262595204576TruSeq Adapter, Index 4 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCCAATCTCGTATG152780.23725245568248982TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATG146340.22725176308794054TruSeq Adapter, Index 4 (97% over 49bp)
CAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC139780.2170647221841761TruSeq Adapter, Index 4 (100% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATG136980.2127165949691547TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAAG131170.20369423099798525TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC112190.17422014009044726TruSeq Adapter, Index 4 (100% over 50bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC106130.16480955047507947TruSeq Adapter, Index 4 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATG100680.15634623143155568TruSeq Adapter, Index 4 (97% over 49bp)
AGAATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT100270.1557095413750704TruSeq Adapter, Index 4 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTG97870.1519825751907663TruSeq Adapter, Index 4 (97% over 49bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA92480.14361243030185009TruSeq Adapter, Index 4 (100% over 40bp)
AAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT92470.14359690127608216TruSeq Adapter, Index 4 (100% over 41bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG87630.1360808528044023TruSeq Adapter, Index 4 (100% over 49bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC83970.13039722937333859TruSeq Adapter, Index 4 (100% over 37bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC82190.1276330627866464TruSeq Adapter, Index 4 (100% over 46bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC77090.11971325964500025TruSeq Adapter, Index 4 (100% over 37bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG76180.11830011830011829TruSeq Adapter, Index 4 (97% over 49bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT70610.10965045094737927TruSeq Adapter, Index 4 (100% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT66010.1025070990941298TruSeq Adapter, Index 4 (100% over 48bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64530.10020880328047564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAATCG18050.066.334811
GAATCGG19450.063.711662
AAATGGG16300.061.41005370
AAAAGGG484050.061.25714570
AGATTCG4750.061.1754381
GATCCGG5050.058.920012
AAACGGG13600.058.67552670
CGGATAC308.385252E-658.33239745
TGATCGG60100.057.9617161
AACAGGG8550.057.71837270
AATAGGG8500.055.99910470
AGATGGA15200.055.9699971
TTGGAAA47250.054.66578762
GATTCGG5350.054.3074652
ACGCATC1750.053.9991337
AGAAGGG4550.053.84528770
AAAGGGG59900.053.58010570
ATATCGC850.053.5447651
ATAAGGG5500.053.45368670
AAGAGGG18400.053.45022670
Produced by FastQC (version 0.11.5)