FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7773395
Sequences flagged as poor quality0
Sequence length76
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG3173534.082553375970217TruSeq Adapter, Index 3 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATG1144721.472612674384873TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATT845631.08785157579153TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATG626470.8059155619906102TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATG619470.7969104876312089TruSeq Adapter, Index 3 (97% over 45bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC386300.496951460719544TruSeq Adapter, Index 3 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTG385180.4955106488220398TruSeq Adapter, Index 3 (97% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG382260.49175424637497517TruSeq Adapter, Index 3 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATT341790.43969205218569235TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATG278170.3578487906506745TruSeq Adapter, Index 3 (97% over 49bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA274770.3534748973903938TruSeq Adapter, Index 3 (100% over 40bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC263370.3388094905765113TruSeq Adapter, Index 3 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATG245640.3160009236633414TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTG229660.29544362534002194TruSeq Adapter, Index 3 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATG220480.283634113537264TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATT211810.27248068572354806TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATT205220.2640030514337686TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTT196010.25215494645518466TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTG195940.25206489571159063TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTG195310.2512544390192445TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATG168880.21725385111653275TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATT156860.20179085200224614TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGTTG137490.1768725248105879TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTT132990.17108354843668694TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATT132480.1704274644476448TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTAAG129160.1661564863229001TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGATG126560.16281174441797952TruSeq Adapter, Index 3 (97% over 45bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA122530.15762739446535265TruSeq Adapter, Index 3 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATT121290.15603220986454439TruSeq Adapter, Index 3 (97% over 42bp)
CAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC120100.1545013472234461TruSeq Adapter, Index 3 (100% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCCTATG119810.15412827985712807TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTT118790.15281611187904384TruSeq Adapter, Index 3 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTG118570.15253309525631978TruSeq Adapter, Index 3 (97% over 42bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG117360.1509765038313375TruSeq Adapter, Index 3 (100% over 37bp)
AAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT107810.13869100952672545TruSeq Adapter, Index 3 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTTTG101450.13050925625161205TruSeq Adapter, Index 3 (97% over 46bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG100210.12891407165080382TruSeq Adapter, Index 3 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTT100010.12865678381196374TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGTATG93390.12014055634635831TruSeq Adapter, Index 3 (97% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84030.10809948548864427No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTT83320.10718611366076211TruSeq Adapter, Index 3 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG79610.10241342425027931TruSeq Adapter, Index 3 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTTTT79400.10214327201949727TruSeq Adapter, Index 3 (97% over 46bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG78790.10135854411103513TruSeq Adapter, Index 3 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAATCG18250.064.270111
AAAAGGG419400.063.17233370
AGATTCG5350.059.5545161
AAATGGG16050.058.00508570
TGATCGG90000.056.798581
ACTGACG1750.055.99889433
AAACGGG9000.055.99889470
GAATCGG22250.054.5940782
AATAGGG9450.054.0730170
CTTGTAA135150.053.78732361
AAAGGGG40300.053.671470
GCTTGTA118800.053.53008760
GCTTTTA32350.053.44561860
TGCTTGT97200.053.03907859
AGATGGA21950.052.957891
TTGTAAA167200.052.45709662
TTTGCTT25550.051.64281557
TTGCTTG18050.051.5779358
AAAACGG38900.051.5542570
AAAATGG70650.051.4214970
Produced by FastQC (version 0.11.5)