FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7648852
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG122090315.961911669881964TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA875971.145230682983538TruSeq Adapter, Index 14 (97% over 38bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC540930.7072041660630902TruSeq Adapter, Index 1 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG536480.7013862995388066TruSeq Adapter, Index 1 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATG385600.5041279397221962TruSeq Adapter, Index 1 (97% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG339530.4438966788741631TruSeq Adapter, Index 1 (100% over 49bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA323600.4230700241029634TruSeq Adapter, Index 1 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATGTCGTATG305260.39909256970850004TruSeq Adapter, Index 1 (97% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG283640.3708268901006321TruSeq Adapter, Index 1 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATG263160.34405163023156937TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG188220.24607614319116125TruSeq Adapter, Index 1 (97% over 49bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA169340.22139270049936904TruSeq Adapter, Index 1 (100% over 37bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA153460.2006314150149591TruSeq Adapter, Index 1 (100% over 40bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT134520.1758695291790193TruSeq Adapter, Index 1 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATG132600.17335934856629465TruSeq Adapter, Index 1 (97% over 49bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG118830.1553566469844102TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCCGATCTCGTATG109410.14304107335323TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATT109160.14271422691928148TruSeq Adapter, Index 1 (97% over 48bp)
AAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT106080.13868747885303573TruSeq Adapter, Index 1 (100% over 41bp)
CAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC100380.13123538015900948TruSeq Adapter, Index 1 (100% over 42bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT96070.125600547637737TruSeq Adapter, Index 1 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC94150.12309036702501239TruSeq Adapter, Index 1 (100% over 50bp)
TTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA93050.12165224271563889TruSeq Adapter, Index 1 (100% over 40bp)
AGAATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT89430.11691950635206434TruSeq Adapter, Index 1 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAAG87230.11404325773331737TruSeq Adapter, Index 1 (97% over 49bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG83950.1097550325199128TruSeq Adapter, Index 1 (97% over 49bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG76540.10006730421767866TruSeq Adapter, Index 1 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAATCG15350.062.499221
GAATCGG16050.061.071122
AAAAGGG517100.060.82742370
AAACGGG15350.058.59815270
TGTAGGG9750.056.739421
TGATCGG74050.056.5980571
AGATTCG6250.056.0212021
AAAGGGG65200.054.80716770
AAATGGG19150.054.46364270
AATAGGG9550.053.8732570
GATTCGG6700.053.293992
ATAAGGG4850.051.95770370
GGATCGG31000.051.9551431
AGATGGA18400.051.7587171
GATCCGG4600.051.749242
GTAGGGA10900.051.7073482
ACGTGTG206900.050.565291
GACGTGT51950.050.278891
GATGGAA19650.049.5262152
AAGAGGG22750.049.07592470
Produced by FastQC (version 0.11.5)