FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4629459
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT45050.049.274731
GCGTGAA63850.041.27575766
CGGCTTC33450.038.9231358
CCGGCTT38250.038.79675357
CGTGAAA72350.038.6517567
GCCGGCT30550.038.60820856
TGCGTGA42300.038.1435865
GGCGTGA24350.037.22731865
CTGCGTG42800.035.4900764
GAAAAAA266200.035.157270
GCGTCGG12600.034.72165360
GTGAAAA101250.034.56733768
GCTTGAA102050.034.53642766
TGCCGGC53750.034.18548655
GCGTGGG14750.034.1689360
TGAAAAA244200.033.0645769
GGGCGTG15850.032.2392264
CTGCTTG81600.031.99702664
GCCCACA13950.031.8756731
TCGGCGT24900.031.6259963
Produced by FastQC (version 0.11.5)