FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218954
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT14200.055.4941021
CGGGAGA1300.035.014323
CACTCTT37850.034.9766771
GCGTGAA12100.034.7092866
GCCCACA4150.034.6009831
ACCGAGC1055.511538E-1033.3319351
TGCGTGA8600.032.96372665
CTGCGTG8100.032.83812364
CGTGAAA13000.032.30632867
GGGAGAT2350.031.2893924
GAAAAAA63050.031.25165670
GCTTGAA43500.030.73433566
TGAAAAA62500.030.0707369
CTTGAAA46500.029.353667
TGCTTGA39950.028.99752465
CTGCTTG40800.028.82231364
GGCGTGA4450.028.31341265
TTGAAAA51150.027.16405568
GTGAAAA17000.026.76357868
TCTGCTT47800.025.04078363
Produced by FastQC (version 0.11.5)