FastQCFastQC Report
Wed 14 Aug 2019
HMWVKAFXY_n01_ncm3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWVKAFXY_n01_ncm3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243878
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT31550.052.715211
GCCGGCT11100.040.3594156
GCGTGAA14950.039.56428566
CCGGCTT16050.039.03334457
CGTGAAA17550.036.69429467
CGGCTTC14500.035.48192258
TGCCGGC20600.034.82927755
CACTCTT76000.033.724011
CGGCTTG12100.032.6851858
GGCGTGA7800.031.85822165
ACCGAGC2200.031.81743251
GTGAAAA33650.031.2028368
CTGCGTG8900.031.06668564
GAAAAAA89200.031.03626470
CGGCGTC9700.031.03019758
TGCGTGA10600.030.37664465
GCTTGGG5850.029.91382660
GCGGCTT10200.029.5091157
GCCCACA7300.029.2582741
GCGTCGG6600.029.1659860
Produced by FastQC (version 0.11.5)