FastQCFastQC Report
Mon 9 May 2022
HMWN5BGXL_n01_aEV_RFP1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWN5BGXL_n01_aEV_RFP1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7530682
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAA149380.19836184823632175No Hit
GCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGT93020.12352134906240896No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG78620.10439957496545466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)