FastQCFastQC Report
Mon 9 May 2022
HMWN5BGXL_n01_aE1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWN5BGXL_n01_aE1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12288459
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCAATCTCGTATGCCGT281680.22922320854063147TruSeq Adapter, Index 13 (96% over 33bp)
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG245320.19963447003403761No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)