FastQCFastQC Report
Mon 9 May 2022
HMWN5BGXL_n01_aCOL4_2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWN5BGXL_n01_aCOL4_2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7325751
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAA172650.23567549593208945No Hit
GCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGT98470.1344162530230689No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG97190.13266899188902273No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA89380.122007968875819No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA77200.10538168714716076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)