FastQCFastQC Report
Sat 7 May 2022
HMWN5BGXL_n01_H2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWN5BGXL_n01_H2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7990839
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT136260.17052026702077216No Hit
GCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAA102880.12874743190295787No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG97000.12138900558502055No Hit
TAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT88340.11055159539567748No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA86220.10789855733546877No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA85410.10688489656718149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)