FastQCFastQC Report
Sat 7 May 2022
HMWN5BGXL_n01_E3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWN5BGXL_n01_E3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6366106
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAA148550.23334515636403166No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA115280.18108400959707552No Hit
GCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGT86200.1354045942684586No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG80510.12646663439157313No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA72790.11433991202785501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)