FastQCFastQC Report
Sat 7 May 2022
HMWN5BGXL_n01_COL4_2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWN5BGXL_n01_COL4_2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6157593
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA145290.2359525873178042No Hit
GCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAA119730.19444286103352398No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG74000.12017682883555311No Hit
GCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGT67270.10924723345631969No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)