FastQCFastQC Report
Fri 6 May 2022
HMWKNBGXL_n01_ZmCA2P10_B73-B_x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWKNBGXL_n01_ZmCA2P10_B73-B_x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11572770
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT760280.6569559405397325TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA622660.5380388619146496TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC488070.4217399982890872TruSeq Adapter, Index 2 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)