FastQCFastQC Report
Thu 18 Jul 2019
HMWG7AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWG7AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10557854
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2194622.078661061234603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG13352.391971E-99.814699145
GAGCACC17553.8198777E-119.124968145
AAGACGC11252.0247899E-69.058603145
CACACGG7250.00175964139.036292145
CACGTCG13009.4892445E-46.719294145
AACCGGC11000.0097064696.6174865145
AGACCGA37253.6379788E-126.253303145
CTCGTAT96900.05.9345675145
CCGACGC18402.0359109E-45.9341598145
CTTGAAA63500.05.846315145
GAGACCG30406.379378E-85.7467647145
GCTTGAA56800.05.4088097140-144
CGGAAGC19100.00208589395.335565145
CCGAGAC31654.7850062E-65.059816145
TCTCGTA96700.05.0140114140-144
ATGCCGT100400.05.0026617145
Produced by FastQC (version 0.11.5)